Studies on the Metabolism of Aspergilli

Part VIII. Relationship between Endogenous Formation of Ammonia and Levels of Mycelial Components

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Studies were made on relationship between endogenous formation of ammonia and levels of mycelial components.
1. It was found that levels of mycelial com-ponents were considerably affected by the concentration of N-, P- and K-sources in cul-ture medium. In the mycelia that were rich in total carbohydrates and glycogen, and less in nitrogenous materials, endogenous forma-tion of ammonia was suppressed or less re-markable.
2. Preincubation of the mycelia with (NH₄)₂SO₄ brought about the reduction of total carbohydrate and glycogen content to stimulate endogenous formation of ammonia.