L-Lysine monohydrochioride and D-glucose were allowed to react in a bicarbonate buffer of pH 8.8 under refluxing The reaction mixture was then chromatographed on a thin-layer plate of Kiesel Gel using n-propanol ethyl-acetate water 25% aqueous ammonia (6:1:2:1 v/v) as a developing agent. Elson-Morgan-reactive spots on the chromatogram were eluted individually, and each of the eluates was incubated with L. bifidus var. pennsylvanicus in a defined medium. A certain fraction on the chromatogram showed remarkably promoting effect on both the acid productivity and the growth of the organism. And such effect of the fraction was much stronger than that of N-acetyl-D-glucosamine which had been known as “Bifidus Factor”
