抄録
The inactivating action of monovalent cation (Na+) and the stabilizing action of divalent cation (Mg2+) on phage were studied using HM 2 phage (group I) of Clostridium saccharoperbutylacetonicum.
The maximum rate of the phage inactivation occured at 0.1M Na+. However, the serum-brocking power (SBP) of phage protein decreased with increasing the concentration of Na+. The inactivation of the phage by Na+ was associated with alteration of its sedimentation rate (s20, w, ), the release of its DNA, and denaturation of its protein. These facts indicate that Na+ causes the destructions of the structures of phage protein and DNA-protein complex. The action of Na+ on the phage could be illustrated by the salting-in and salting-out effects of protein of the phage by Na+.
