Abstract
Miniaturized bioassay technique, mini-test, was developed to estimate biologically available energy of a sample of only 5_??_50g. Chicks were previously starved to keep body size minimum, then given either standard diets of three energy levels or test diets containing 5% of the sample for 6 days. Available energy of the sample was estimated on the standard curve showing linear relationship between dietary energy level and response of the chicks on the standard diets.
Reliability of the estimate by this mini-test was discussed based on linearity of the standard curve, 95% confidence interval of the estimate and available energy of 2 known carbohydrates estimated by this mini-test.
