抄録
The molecular weight of the yeast tannase [E. C. 3. 1. 1. 20, tannin acyl-hydrolase] of Candida sp. was determined to be 250, 000 by gel filtration on Sephadex G-200. The enzyme was dissociable into two identical subunits with molecular weight of 120, 000 on SDS-polyacrylamide gel electrophoresis. The amino acid analysis revealed that the enzyme consisted of 786 amino acid residues per protein molecule. The polypeptide moiety of the enzyme was 38% by the Lowry-Folin reaction and 35% by the amino acid analysis. The enzyme contained 62% neutral sugars, which were identified as mannose and galactose on cellulose thin-layer chromatogram and 2.2% hexosamines.
