抄録
To clarify the mechanism of electrochemical insolubilization of DNA, metal contents combined to DNA were examined, and the binding manner of the metals to DNA was pre-sumed from the sedimentation coefficient and ultraviolet absorption spectra of DNA insolu-bilized by electrolysis.
Copper content increased with an increase in current passed and decreased with an increase in temperature of DNA solution, but was not affected by pH.
The sedimentation coefficient revealed a slight cleavage of DNA.
The ultraviolet absorption maxima shifted to a shorter wavelength with an increase in copper content, and to the longer side with an increase in temperature of DNA solution.
On the other hand, nickel showed a similar behavior to copper except that their combined content was affected by pH. Iron content was only a paucity compared with the amounts of copper and nickel.
From these results, it was presumed that copper combined with the phosphate group of DNA when Cu-DNA was prepared from native DNA and with the bases of that from denatured DNA. In addition, it seemed that the binding manner of metals to DNA differed from one another with the kind of metal.
