Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Enzymatic Synthesis of 4-O-α-D-Glucopyranosyl-moranoline
Yohji EZURE
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1985 年 49 巻 7 号 p. 2159-2165

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A transglycosylation reaction with moranoline (1-deoxynojirimycin) was carried out with α-cyclodextrin as the glucose donor and Bacillus macerans amylase as cyclodextrin glycosyltransferase [EC 2.4.1.19]. The resultant transglycosylation products were hydrolyzed by glucoamylase [EC 3.2.1.3] from Rhizopus niveus. The hydrolyzate (the transglycosylation product of the lowest molecular weight) was isolated and the structure was found by physico-chemical methods to be 4-O-α-D-glucopyranosyl-moranoline.
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