Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Soluble and Bound Forms of Aminopeptidase in Hen's Egg Yolk
Eiji ICHISHIMAYouhei YAMAGATAHideki CHIBAKazuyo SAWAGUCHITomoaki TANAKA
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ジャーナル フリー

1989 年 53 巻 7 号 p. 1867-1872

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抄録
An aminopeptidase. Ey, is a newly discovered enzyme of hen's egg yolk. About 95 % of the total activity was found in the yolk plasma fraction, with most of the remainder in the yolk granule fraction. The highly purified fraction from plasma showed about 13, 000-fold higher specific activities than that of yolk plasma. Polyacrylamide gel electrophoresis revealed that the enzyme was homogeneous. The bound enzyme in the granule was extracted with 3 % NaCl and was partially purified. The soluble and bound forms of aminopeptidase differ in solubility, molecular weight, heat stability, optimal pH, and Km.
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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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