Production of N-Acetylglucosamine Deacetylase by Vibrio cholerae Non-O1

Abstract

Vibrio cholerae non-O1 (1148A) produced β-N-acetylglucosaminidase and N-acetylglucosamine (GlcNAc) deacetylase intracellularly when grown in chitin or GlcNAc containing medium. It also secreted chitinase only in the chitin-containing medium. The partially purified GlcNAc deacetylase deacetylated GlcNAc but not chitin oligosaccharides, the dimer to hexamer of GlcNAc. We also detected the reaction product by capillary electrophoresis.