Bioscience and Microflora
Online ISSN : 1349-8355
Print ISSN : 1342-1441
ISSN-L : 1342-1441
A Modified Continuous Flow Culture System for Studying Microbial Interactions Related to the Gastro-Intestinal Tract: Application of the Probiotic Organism Enterococcus faecium SF68
Margaretha DU TOITSilvia KNOPFELFrank A. SKÖWRONEKIngrid SPECHTWolfgang BÖCKELERWilhelm H. HOLZAPFEL
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1998 年 17 巻 1 号 p. 41-47

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A modified continuous flow system was used to test the regulative effect of Enterococcus faecium SF68 on single strain cultures of Lactobacillus reuteri and Lactobacillus acidophilus representative of the autochthonous intestinal microflora. Experiments were conducted in duplicate using serially arranged fermentation cells. The second (A) and third (B) cells were filled with agar blocks containing an immobilized culture of either L. reuteri or L. acidophilus. The data obtained confirmed observations from previous clinical tests on the influence of E. faecium SF68 in the treatment of intestinal disorders. In our in vitro investigations with the continuous flow system E. faecium SF68 accelerated the restoration of the L. reuteri and L. acidophilus populations when applied after antibiotic treatment. The application of E. faecium SF68 together with the antibiotic had a protective effect on the L. reuteri and L. acidophilus populations, as cell numbers decreased at a significantly lower rate compared to the control unit.
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