DETECTION OF NUCLEIC ACID COMPONENTS ON PAPER CHROMATOGRAM
1954 年 41 巻 4 号 p. 457-462
詳細
1954 年 41 巻 4 号 p. 457-462
The spots of nucleic acid components on paper chromatogram were detected by an ultraviolet detector. This detector consists of a low voltage mercury lamp (Matsuda's sterilization lamp GL-15, 100v., 15w.) as the light source and a solution of nickel and cobalt sulfate described by Markham and Smith as the filter. In this paper, the apparatus, the spectrophotometric research in the detector and Rf values of nucleic acid components were described.
The lower limit of detection with this detector was 1 to 5γ of each compound.
This work was aided by a grant from the Scientific Research Fund of the Ministry of Education.
