Autoradiographic Study of Developmental Sequence of New Odontoblasts in Rat Incisor Pulp after Colchicine Administration

抄録

The sequence of newly formed odontoblasts induced by colchicine was studied by an autoradiographic technique using 3H-proline in the rat incisor pulp. Four days after colchicine administration, marked cell proliferation and revascularization were observed in the necrotic pulp tissue. On the 6th day after colchicine administration, an organic matrix heavily labeled with 3H-prolin appeared in the cell proliferation zone and vascularity was observed adjacent to the organic matrix. The number of silver grains over the organic matrix was significantly greater than that over the other pulp tissue and that over the cell proliferation zone after 4 days. On the 8th day after colchicine administration, odontoblast-like cells were seen in the area of vascularity in contact with the organic matrix. The number of silver grains over odontoblast-like cells was significantly greater than that over the organic matrix on the 8th day or on the 6th day. On the 10th day after colchicine administration, new odontoblasts and predentine were clearly ovrerved in contact with the organic matrix. The number of silver grains over the new odontoblasts and pre-dentine was also heavily labeled with 3H-prolin. These findings suggest that new odontoblasts develop from perivascular mesenchymal stem cells during reparative dentinogenesis after colchicine destruction.