総説
トマトのウイルス抵抗性タンパク質によるウイルス増殖阻害とウイルスによる耐性獲得の構造基盤
2016 年 56 巻 1 号 p. 009-012
詳細
2016 年 56 巻 1 号 p. 009-012
The Tm-1 gene of tomato encodes a 754-aa protein that binds tomato mosaic virus (ToMV) replication proteins and inhibits viral RNA replication. We determined a crystal structure of complex of an N-terminal fragment of Tm-1 and helicase domain of ToMV (ToMV-Hel). The residues in ToMV-Hel that are changed in resistance-breaking mutants are directly involved in the interaction. The positively selected region of Tm-1 formed the binding surface with ToMV-Hel. Thus, the antagonistic coevolution between a resistance protein and a viral protein has occurred at the interaction interface on both sides.
