2002 年 25 巻 p. 1-7
A high-density genetic linkage map with PCR-based markers provides a simple and fast system for scanning genome. One hundred and six newly developed RAPD markers were assigned to a RFLP framework map previously constructed using the RI lines of a cross between rice varieties of Asominori and IR24. From a total of 306 RAPD primers screened, 119 reproducible RAPDs were detected. Of the 119 RAPDs, 95 showed a normal Mendelian segregation at a 1:1 ratio in the RI lines. One hundred and six out of the 119 RAPD markers were assigned to the 12 chromosomes and the rest (13 markers) did not show a significant linkage with the RFLP markers on the framework map. ‘The RAPD markers were distributed widely over the rice genome, but some of them were placed by clustering in particular chromosomal regions. This combined RFLP/RAPD genetic linkage map could be an important tool for marker-assisted selection in a breeding program. Furthermore, this map will greatly facilitate quantitative traits analysis and map-based cloning of agronomically important genes.
