Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Notes
Effects of Adenine-Nucleotides on the Sequence-Specificity of Origin Recognition Complex-Binding to DNA
Hitomi TakenakaNaoko TakahashiJong-Ryul LeeMasaki MakiseYoshihiro YamaguchiTomofusa TsuchiyaTohru Mizushima
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2002 年 25 巻 5 号 p. 652-655

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We examined effects of adenine-nucleotides on the sequence-specificity of origin recognition complex (ORC)-binding to DNA, using a gel electrophoretic mobility shift assay. The sequence-specific DNA binding of ORC was observed in the presence of ATP or ATP-γ-S but not in the presence of adenosine 5′-diphosphate (ADP) or in the absence of any adenine-nucleotides. In contrast, the sequence-independent DNA binding of ORC was observed under any one of these conditions. These results suggest that ATP increases the sequence-specificity of ORC-binding to DNA. In relation to the requirement for incubation at high temperature and inhibition by cardiolipin, there was no significant difference between the sequence-specific and the sequence-independent DNA binding activities of ORC.
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© 2002 The Pharmaceutical Society of Japan
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