Delivery of Condensed DNA by Liposomal Non-viral Gene Delivery System into Nucleus of Dendritic Cells

Takashi Nakamura; Rumiko Moriguchi; Kentaro Kogure; Arisa Minoura; Tomoya Masuda; Hidetaka Akita; Kazunori Kato; Hirofumi Hamada; Masaharu Ueno; Shiroh Futaki; Hideyoshi Harashima

doi:10.1248/bpb.29.1290

Notes

Delivery of Condensed DNA by Liposomal Non-viral Gene Delivery System into Nucleus of Dendritic Cells

Takashi Nakamura, Rumiko Moriguchi, Kentaro Kogure, Arisa Minoura, Tomoya Masuda, Hidetaka Akita, Kazunori Kato, Hirofumi Hamada, Masaharu Ueno, Shiroh Futaki, Hideyoshi Harashima

Author information

Takashi Nakamura
Graduate School of Pharmaceutical Sciences, Hokkaido University
Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)
Rumiko Moriguchi
Graduate School of Pharmaceutical Sciences, Hokkaido University
Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)
Kentaro Kogure
Graduate School of Pharmaceutical Sciences, Hokkaido University
Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)
Arisa Minoura
Graduate School of Pharmaceutical Sciences, Hokkaido University
Tomoya Masuda
Graduate School of Pharmaceutical Sciences, Hokkaido University
Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)
Hidetaka Akita
Graduate School of Pharmaceutical Sciences, Hokkaido University
Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)
Kazunori Kato
Department of Molecular Medicine, Sapporo Medical University
Hirofumi Hamada
Department of Molecular Medicine, Sapporo Medical University
Masaharu Ueno
Faculty of Pharmaceutical Sciences, University of Toyama
Shiroh Futaki
Institute for Chemical Research, Kyoto University
Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST)
Hideyoshi Harashima
Graduate School of Pharmaceutical Sciences, Hokkaido University
Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)

Keywords: non-viral gene delivery system, non-dividing cell, nuclear localization signal, nuclear transfer

JOURNAL FREE ACCESS

2006 Volume 29 Issue 6 Pages 1290-1293

DOI https://doi.org/10.1248/bpb.29.1290

Details

Abstract

In this study, we developed novel double-membranous non-viral gene delivery system modified with SV-40 T antigen-derived nuclear localization signal (NLS-DMEND) for delivery of luciferase plasmid DNA to nucleus of non-dividing mouse bone marrow-derived dendritic cells (BMDC). Intracellular trafficking and gene expression of NLS-DMEND in the BMDC were evaluated. Condensed DNA was observed in the nucleus by confocal laser scanning microscopy, and the NLS-DMEND induced significant luciferase activity in the BMDC. It was suggested that the condensed DNA particle transferred into nucleus via energy dependent manner, since the nuclear transfer was inhibited by metabolic inhibitors. In conclusion, condensed plasmid DNA was delivered into the nucleus of non-dividing BMDC by NLS-DMEND.

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