Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Silencing of Exogenous DNA in Cultured Cells
Hiroshi OchiaiHideyoshi HarashimaHiroyuki Kamiya
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JOURNAL FREE ACCESS

2006 Volume 29 Issue 6 Pages 1294-1296

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Abstract

The intranuclear disposition of exogenous DNA is highly important for the therapeutic effects of the administrated DNA. Naked luciferase-plasmid DNA was transfected into cultured cells including HeLa by electroporation, and the amounts of intranuclear plasmid DNA and luciferase were quantitated at various time points. Decrease in expression efficiency from one copy of the exogenous DNA over time occurred as the case of mouse liver, and its degrees varied between cell lines. These results suggest that exogenous DNA is ‘silenced’ in the cultured cells as well as in mouse hepatocytes.

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© 2006 The Pharmaceutical Society of Japan
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