Biotin–Protein Ratios and Stability of Biotinylated Immunoglobulins as Standards for the Quantitation of Biotin-Binding Immunoglobulins

Ayaka Yazawa; Kyoko Fukuoka; Hidemi Honda; Toru Fukui; Makoto Muratsugu

doi:10.1248/bpb.29.1480

Notes

Biotin–Protein Ratios and Stability of Biotinylated Immunoglobulins as Standards for the Quantitation of Biotin-Binding Immunoglobulins

Ayaka Yazawa, Kyoko Fukuoka, Hidemi Honda, Toru Fukui, Makoto Muratsugu

著者情報

キーワード: biotinylated protein, biotin–protein ratio, stability, competitive binding assay, bioassay, biotin-binding immunoglobulin

ジャーナルフリー

2006 年 29 巻 7 号 p. 1480-1482

DOI https://doi.org/10.1248/bpb.29.1480

詳細

抄録

Biotin-binding IgG in human sera was quantitated using F(ab')₂anti-human IgG-coated multiwell microplates (Muratsugu, M. et al. 2003, Biol. Pharm. Bull., 26, 1605—1608). The biotin–protein ratio of biotinylated IgG, which was used as standard in the assay, was very important to quantitate the level of biotin-binding IgG. We investigated a synthesis method of biotinylated human immunoglobulins, how to determine the biotin–protein ratio of the biotinylated proteins, and their stability to prepare standards for measuring biotin-binding IgG, IgA, and IgM.

責任著者(Corresponding author)

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