DNA Authentication of Plantago Herb Based on Nucleotide Sequences of 18S–28S rRNA Internal Transcribed Spacer Region

Fatma Pinar Sahin; Hiromi Yamashita; Yahong Guo; Kazuyoshi Terasaka; Toshiya Kondo; Yutaka Yamamoto; Hiroshi Shimada; Masao Fujita; Takeshi Kawasaki; Eiji Sakai; Toshihiro Tanaka; Yukihiro Goda; Hajime Mizukami

doi:10.1248/bpb.30.1265

Regular Articles

DNA Authentication of Plantago Herb Based on Nucleotide Sequences of 18S–28S rRNA Internal Transcribed Spacer Region

Fatma Pinar Sahin, Hiromi Yamashita, Yahong Guo, Kazuyoshi Terasaka, Toshiya Kondo, Yutaka Yamamoto, Hiroshi Shimada, Masao Fujita, Takeshi Kawasaki, Eiji Sakai, Toshihiro Tanaka, Yukihiro Goda, Hajime Mizukami

著者情報

キーワード: Plantago Herb, DNA authentication, internal transcribed spacer, internal transcribed spacer (ITS), ribosomal DNA

ジャーナルフリー

2007 年 30 巻 7 号 p. 1265-1270

DOI https://doi.org/10.1248/bpb.30.1265

詳細

抄録

Internal transcribed spacer (ITS) regions of nuclear ribosomal RNA gene were amplified from 23 plant- and herbarium specimens belonging to eight Plantago species (P. asiatica, P. depressa, P. major, P. erosa, P. hostifolia, P. camtschatica, P. virginica and P. lanceolata). Sequence comparison indicated that these Plantago species could be identified based on the sequence type of the ITS locus. Sequence analysis of the ITS regions amplified from the crude drug Plantago Herb obtained in the markets indicated that all the drugs from Japan were derived from P. asiatica whereas the samples obtained in China were originated from various Plantago species including P. asiatica, P. depressa, P. major and P. erosa.

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