Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Kaempferol Attenuates 2-Deoxy-D-ribose-Induced Oxidative Cell Damage in MC3T3-E1 Osteoblastic Cells
Kwang Sik SuhEun-Mi ChoiMikwang KwonSuk ChonSeungjoon OhJeong-Taek WooSung Woon KimJin-Woo KimYoung Seol Kim
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2009 Volume 32 Issue 4 Pages 746-749


Reducing sugar, 2-deoxy-D-ribose (dRib), produces reactive oxygen species through autoxidation and protein glycosylation and causes osteoblast dysfunction. Kaempferol, a natural flavonoid, was investigated to determine whether it could influence dRib-induced cellular dysfunction and oxidative cell damage in the MC3T3-E1 mouse osteoblastic cell line. Osteoblastic cells were treated with 30 mM dRib in the presence or absence of kaempferol (10−9—10−5 M) and markers of osteoblast function and lipid peroxidation were subsequently examined. Kaempferol (10−9—10−5 M) significantly inhibited the dRib-induced decrease in growth of MC3T3-E1 osteoblastic cells. In addition, treatment with kaempferol resulted in a significant elevation of alkaline phosphatase (ALP) activity, collagen content, and mineralization in the cells. Treatment with kaempferol increased osteoprotegerin (OPG) secretion and decreased malondialdehyde (MDA) contents of MC3T3-E1 osteoblastic cells in the presence of 30 mM dRib. Taken together, these results suggest that kaempferol inhibits dRib-induced osteoblastic cell damage and may be useful for the treatment of diabetes-related bone disease.

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© 2009 The Pharmaceutical Society of Japan
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