β-Galactosidases from Jack Bean and Streptococcus Have Different Cleaving Abilities towards Fucose-Containing Sugars

Tomoharu Takeuchi; Ken-ichi Sugiura; Kazusa Nishiyama; Hideyo Takahashi; Hideaki Natsugari; Yoichiro Arata; Shunji Natsuka; Ken-ichi Kasai

doi:10.1248/bpb.34.567

Note

β-Galactosidases from Jack Bean and Streptococcus Have Different Cleaving Abilities towards Fucose-Containing Sugars

Tomoharu Takeuchi, Ken-ichi Sugiura, Kazusa Nishiyama, Hideyo Takahashi, Hideaki Natsugari, Yoichiro Arata, Shunji Natsuka, Ken-ichi Kasai

著者情報

キーワード: β-galactosidase, galactose β1-4 fucose, Protostomia, Caenorhabditis elegans

ジャーナルフリー

2011 年 34 巻 4 号 p. 567-569

DOI https://doi.org/10.1248/bpb.34.567

詳細

抄録

We examined the sugar-cleaving abilities of β-galactosidases from jack bean and Streptococcus towards sugars containing fucose residues, and found that jack bean β-galactosidase has an ability to cleave the β1-3 linkage between galactose (Gal) and fucose (Fuc) residues, but not β1-4 linkage. On the other hand, streptococcal β-galactosidase was found to cleave the linkage in both Galβ1-4Fuc and Galβ1-3Fuc disaccharide units. Such a difference in sugar-cleaving abilities between these 2 β-galactosidases will be useful for structural analysis of glycans, especially those from species belonging to Protostomia, such as Caenorhabditis elegans.

責任著者(Corresponding author)

J-STAGEへの登録はこちら（無料）