Comparison of Human Selenoprotein P Determinants in Serum between Our Original Methods and Commercially Available Kits

Yoshiro Saito; Hirofumi Misu; Hiroaki Takayama; Shin-ichiro Takashima; Soichiro Usui; Masayuki Takamura; Shuichi Kaneko; Toshinari Takamura; Noriko Noguchi

doi:10.1248/bpb.b18-00046

Notes

Comparison of Human Selenoprotein P Determinants in Serum between Our Original Methods and Commercially Available Kits

Yoshiro Saito , Hirofumi Misu, Hiroaki Takayama, Shin-ichiro Takashima, Soichiro Usui, Masayuki Takamura, Shuichi Kaneko, Toshinari Takamura, Noriko Noguchi

Author information

Yoshiro Saito Corresponding author
Systems Life Sciences Laboratory, Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University
Hirofumi Misu
Department of Endocrinology and Metabolism, Kanazawa University Graduate School of Medical Sciences
PRESTO, Japan Science and Technology Agency
Hiroaki Takayama
Department of Endocrinology and Metabolism, Kanazawa University Graduate School of Medical Sciences
Department of Disease Control and Homeostasis, Kanazawa University Graduate School of Medical Sciences
Shin-ichiro Takashima
Department of Disease Control and Homeostasis, Kanazawa University Graduate School of Medical Sciences
Soichiro Usui
Department of Disease Control and Homeostasis, Kanazawa University Graduate School of Medical Sciences
Masayuki Takamura
Department of Disease Control and Homeostasis, Kanazawa University Graduate School of Medical Sciences
Shuichi Kaneko
Department of Disease Control and Homeostasis, Kanazawa University Graduate School of Medical Sciences
Toshinari Takamura
Department of Endocrinology and Metabolism, Kanazawa University Graduate School of Medical Sciences
Noriko Noguchi
Systems Life Sciences Laboratory, Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University

Keywords: selenoprotein P, monoclonal antibody, enzyme-linked immunosorbent assay (ELISA), sol particle homogeneous immunoassay (SPIA), commercially available kit

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Supplementary material

2018 Volume 41 Issue 5 Pages 828-832

DOI https://doi.org/10.1248/bpb.b18-00046

Details

Abstract

Selenoprotein P (SeP) is a selenium (Se)-rich extracellular protein. SeP is identified as a hepatokine, causing insulin resistance in type 2 diabetes. Thus, the measurement of SeP in serum has received much attention, and several enzyme-linked immunosorbent assay (ELISA) kits for SeP determination are now commercially available. In the present study, we determined the serum SeP levels by our original ELISA and sol particle homogeneous immunoassay (SPIA) methods and also by commercially available kits, and these determinants were compared. We found a kit-dependent correlation of the determinants with our methods. These results suggest that the selection of kit is critical for comparison with our previous reports and for discussing the relationship between the serum SeP levels and disease condition.

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