Nucleosides and Nucleotides. CXIX. Inhibition of DNA-Cytosine Methylase Hha I by a Self-Complementary Oligonucleotide Containing 5-Fluorocytosine

Akira ONO; Yuka MATSUO; Akira MATSUDA; Tohru UEDA

doi:10.1248/bpb.16.529

小野晶, 松尾由佳, 松田彰, 上田亨

著者情報

キーワード: oligonucleotide synthesis, 5-fluorocytosine, DNA-methylase, Hha I, inhibition, restriction enzyme

ジャーナルフリー

1993 年 16 巻 6 号 p. 529-533

DOI https://doi.org/10.1248/bpb.16.529

詳細

抄録

A self-complementary decadeoxyribonucleotide, 5'd(GAAGFGCTTC)3', containing 5-fluorocytosine (F) in substitution for cytosine at the methylation site of DNA-cytosine methylase Hha I (MHha I) has been synthesized. MHha I was inhibited by the pre-incubation of the enzyme with d(GAAGFGCTTC).

責任著者(Corresponding author)

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