Photoaffinity Labeling of Tumor Promoter-Binding Protein (CN-TPBP) and Preparation of Affinity Sorbent Gels

Akihiko AZUMA; Yunike ANDO; Shigeo IWASAKI; Yuichi HASHIMOTO; Mitsuhiro YAMAGUCHI; Shunji TAKEHANA; Hiroshi FUKASAWA; Yasuyuki ENDO; Koichi SHUDO

doi:10.1248/bpb.20.6

Akihiko AZUMA, Yunike ANDO, Shigeo IWASAKI, Yuichi HASHIMOTO, Mitsuhiro YAMAGUCHI, Shunji TAKEHANA, Hiroshi FUKASAWA, Yasuyuki ENDO, Koichi SHUDO

著者情報

Akihiko AZUMA
Yunike ANDO
Shigeo IWASAKI
Yuichi HASHIMOTO
Mitsuhiro YAMAGUCHI
Shunji TAKEHANA
Hiroshi FUKASAWA
Yasuyuki ENDO
Koichi SHUDO
著者所属：
Institute of Molecular & Cellular Biosciences, University of Tokyo
Institute of Molecular & Cellular Biosciences, University of Tokyo
Institute of Molecular & Cellular Biosciences, University of Tokyo
Institute of Molecular & Cellular Biosciences, University of Tokyo
Faculty of Pharmaceutical Sciences, University of Tokyo
Faculty of Pharmaceutical Sciences, University of Tokyo
Faculty of Pharmaceutical Sciences, University of Tokyo
Faculty of Pharmaceutical Sciences, University of Tokyo
Faculty of Pharmaceutical Sciences, University of Tokyo

キーワード: phorbol, tumor promoter, binding protein, affinity gel, oxysterol

ジャーナルフリー

1997 年 20 巻 1 号 p. 6-9

DOI https://doi.org/10.1248/bpb.20.6

詳細

抄録

Cytosolic-nuclear tumor promoter-specific binding protein (CN-TPBP) was photoaffinity-labeled with a specific ligand, 3β, 5α-dihydroxycholestan-6-one (YS-64). Analysis by ODS-HPLC of peptide fragments obtained from the labeled CN-TPBP by trypsinization indicated the existence of a single specifically labeled site. Affinity gels for the purification of CN-TPBP were then prepared. As ligands for the affinity gels, 12-O-tetradecanoylphorbol 13-acetate (TPA), a typical phorbol-type tumor promoter, and benzolactam-V8-310 (BL-V8-310), a structural/biological mimic of teleocidin-class tumor promoters, were adopted. The use of these gels afforded a protein that showed a single band of 58 kDa on SDS-PAGE.

責任著者(Corresponding author)

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