Inhibition of the Metallo-β-lactamase Produced from Serratia marcescens by Thiol Compounds

Masafumi GOTO; Tatsuya TAKAHASHI; Fumiko YAMASHITA; Ako KOREEDA; Hiromasa MORI; Michio OHTA; Yoshichika ARAKAWA

doi:10.1248/bpb.20.1136

Masafumi GOTO, Tatsuya TAKAHASHI, Fumiko YAMASHITA, Ako KOREEDA, Hiromasa MORI, Michio OHTA, Yoshichika ARAKAWA

著者情報

キーワード: metallo-β-lactamase, inhibition, inhibitor, thiol compound, mercaptoacetic acid

ジャーナルフリー

1997 年 20 巻 11 号 p. 1136-1140

DOI https://doi.org/10.1248/bpb.20.1136

詳細

抄録

Low molecular weight thiol compounds have been found to be strong inhibitors of metallo-β-lactamase (IMP-1) produced by Serratia marcescens TN9106, which was expressed by Echerichia coli JM109 cells. Mercaptoacetic acid and 2-mercaptopropionic acid strongly and competitively inhibited IMP-1 with K_i of 0.23 and 0.19 μM, respectively. 2-Mercaptoethanol reversibly inhibited IMP-1 but did not show simple competitive inhibition.

責任著者(Corresponding author)

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