Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Inhibitory Effect of Calcium-binding Protein Regucalcin on Protein Kinase C Activity in Rat Renal Cortex Cytosol
Hideyuki KUROTAMasayoshi YAMAGUCHI
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1998 Volume 21 Issue 4 Pages 315-318

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Abstract

The effect of regucalcin, a calcium-binding protein, on Ca2+ -and phospholipid-dependent protein kinase (protein kinase C) activity in the cytosol of rat kidney cortex was investigated. With increasing concentratoins of Ca2+, phosphatidylserine or dioctanoylglycerol in the reaction mixture, regucalcin (10-8M) caused a remarakble inhibition of protein kinase C activity. Regucalcin did not have a significant effect on protein kinase C activity in the presence of phosphatidylserine or dioctanoylglycerol without Ca2+ addition. Moreover, regucalcin significantly inhibited phorbol 12-myristate 13-acetate (PMA)-increased protein kinase C activity. Meanwhile, staurosporine (10-9M) caused a signidficant inhibition of protein kinase C activity. This inhibition was further enhanced by regucalcin addition. Regucalcin itself did not have protein kinase activity in either the presence or the absence ot both Ca2+ and phospholipids. These results clearly indicate that regucalcin has an inhibitory effect on protein kinase C activity in the cytosol of rat kidney cortex. This inhibitory effect may be partly due to the regucalcin-induced Ca2+ binding.

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