Live Detection of Bioluminescent Preimplantation Bovine Embryos

Tatsuo MURAMATSU; Kazuteru FUKAZAWA; Akihiro NAKAMURA

doi:10.2508/chikusan.70.437

Tatsuo MURAMATSU, Kazuteru FUKAZAWA, Akihiro NAKAMURA

著者情報

キーワード: Early screening, Bovine oocytes, Firefly luciferase gene, LacZ gene, Bioluminescence

ジャーナルフリー

1999 年 70 巻 6 号 p. 437-443

DOI https://doi.org/10.2508/chikusan.70.437

詳細

Published: 1999/11/25 Received: 1999/05/07 Available on J-STAGE: 2008/03/10 Accepted: 1999/08/16 Advance online publication: - Revised: -
訂正情報
訂正日: 2008/03/10 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : 1) Bowen RA, Reed ML, Schnieke A, Shidel GE Jr, Stacey A, Thomas WK, Kajikawa O. Transgenic cattle resulting from biopsied embryos: expression of c-ski in a transgenic calf. Biology of Reproduction, 50: 664-648. 1994.
2) Burdon TG, Wall RJ. Fate of microinjected genes in preimplantation mouse embryos. Molecular Reproduction and Development, 33: 436-442. 1992.
3) Cousens C, Carver AS, Wilmut I, Colman A, Garner I, O'Neill GT. Use of PCR based methods for selection of integrated transgenes in preimplantation embryos. Molecular Reproduction and Development, 39: 384-391. 1994.
4) Eystone J, Jones JM, First NL. Some factors affecting the efficacy of oviduct tissue-conditioned medium for the culture of early bovine embryos. Journal of Reproduction and Fertility, 92: 59-64. 1991.
5) Hamano H, Kuwayama M. Effect of supplementing a culture medium with calf serum on in vitro maturation and development of bovine oocytes after in vitrofertilization. Journal of Reproduction and Technolo-gy, 14: 6-16 1992. (in Japanese)
6) Ikawa M, Kominami K, Yoshimura Y, Tanaka K,Nishimune Y, Okabe M. A rapid and non-invasiveselection of transgenic embryos before implantationusing green fluorescent protein (GFP). FEBS Letter,35: 125-128. 1995.
7) Janne J, Hyttinen J-M, Peura T, Tolvanen M, Alhohen L, Halmekyto M. Transgenic animals as bioproducers of therapeutic proteins. Annual Medicine, 24: 273-280. 1992.
8) Lin S, Yang S, Hopkins N. LacZ expression in germline transgenic zebrafish can be detected in living embryos. Developmental Biology, 161: 77-83. 1994.
9) Matsumoto K, Anzai M, Nakagata N, Takahashi A, Takahashi Y, Miyata, K. Onset of paternal gene activation in early mouse embryos fertilized with transgenic mouse sperm. Molecular Reproduction and Development, 39: 136-140. 1994.
10) Muramatsu T, Nakamura A. Production of transgenic cattle as an animal bioreactor: current status and perspective. Trends in Comparative Biochemistry and Physiology, 3: 9-21. 1997.
11) Nakamura A, Okumura J, Muramatsu T. Early screening of gene expression of SV40 driven lacZ introduced into bovine embryos. Asian-Australasian Journal of Animal Science, 8: 449-454. 1995.
12) Nakamura A, Okumura J, Muramatsu T. Quantitative analysis of transcriptional activity of viral and hybrid promoters in bovine preimplantation embryos. Molecular Reproduction and Development, 49: 368-373. 1998.
13) Nakamura A, Okumura J, Muramatsu T. Quick detection of firefly luciferase gene expression in live developing bovine embryos by photoncounting. Asian-Australasian Journal of Animal Science, 11: 498-502. 1998.
14) Nolan GP, Fiering S, Nicolas JF, Herzenberg LA. Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-D-galactosidase activity after transduction of Escherichia coli lacZ. Proceedings of National Academy of Sciences USA, 85: 2603-2607. 1988.
15) Peters KG, Rao PS, Bell BS, Kindman LA. Green fluorescent fusion proteins: powerful tools for monitoring protein expression in live zebrafish embryos. Developmental Biology, 171: 252-257. 1995.
16) SAS Institute. SAS User's Guide: Statistics. Version 5 edition, SAS Institute, Cary, NC. 1995.
17) Schnieke AE, Kind AJ, Ritchie WA, Mycock K, Scott AR, Ritchie M, Wilmut I, Colman A, Campbell KHS. Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts. Science, 278: 2130-2133. 1997.
18) Thompson EM, Adenot P, Tsuji FI, Renard J-P. Real time imaging of transcriptional activity in live mouse preimplantation embryos using a secreted luciferase. Proceedings of National Academy of Sciences USA, 92: 1317-1321. 1995.
19) Thompson JF, Hayes LS, Lloyd DB. Modulation of firefly luciferase stability and impact on studies of gene regulation. Gene, 103: 171-177. 1991.
20) Vilotte JL, Soulier S, Mercier JC, Gaye P, HueDelahaie D, Furet JP. Complete nucleotide sequence of bovine alpha-lactalbumin gene: comparison with its rat counterpart. Biochimie, 69: 609-620. 1987.

Right : 1) Bowen RA, Reed ML, Schnieke A, Shidel GE Jr, Stacey A, Thomas WK, Kajikawa O. Transgenic cattle resulting from biopsied embryos: expression of c-ski in a transgenic calf. Biology of Reproduction, 50: 664-648. 1994.
2) Burdon TG, Wall RJ. Fate of microinjected genes in preimplantation mouse embryos. Molecular Reproduction and Development, 33: 436-442. 1992.
3) Cousens C, Carver AS, Wilmut I, Colman A, Garner I, O'Neill GT. Use of PCR based methods for selection of integrated transgenes in preimplantation embryos. Molecular Reproduction and Development, 39: 384-391. 1994.
4) Eystone J, Jones JM, First NL. Some factors affecting the efficacy of oviduct tissue-conditioned medium for the culture of early bovine embryos. Journal of Reproduction and Fertility, 92: 59-64. 1991.
5) Hamano H, Kuwayama M. Effect of supplementing a culture medium with calf serum on in vitro maturation and development of bovine oocytes after in vitrofertilization. Journal of Reproduction and Technolo-gy, 14: 6-16 1992. (in Japanese)
6) Ikawa M, Kominami K, Yoshimura Y, Tanaka K,Nishimune Y, Okabe M. A rapid and non-invasiveselection of transgenic embryos before implantationusing green fluorescent protein (GFP). FEBS Letter,35: 125-128. 1995.
7) Janne J, Hyttinen J-M, Peura T, Tolvanen M, Alhohen L, Halmekyto M. Transgenic animals as bioproducers of therapeutic proteins. Annual Medicine, 24: 273-280. 1992.
8) Lin S, Yang S, Hopkins N. LacZ expression in germline transgenic zebrafish can be detected in living embryos. Developmental Biology, 161: 77-83. 1994.
9) Matsumoto K, Anzai M, Nakagata N, Takahashi A, Takahashi Y, Miyata, K. Onset of paternal gene activation in early mouse embryos fertilized with transgenic mouse sperm. Molecular Reproduction and Development, 39: 136-140. 1994.
10) Muramatsu T, Nakamura A. Production of transgenic cattle as an animal bioreactor: current status and perspective. Trends in Comparative Biochemistry and Physiology, 3: 9-21. 1997.
11) Nakamura A, Okumura J, Muramatsu T. Early screening of gene expression of SV40 driven lacZ introduced into bovine embryos. Asian-Australasian Journal of Animal Science, 8: 449-454. 1995.
12) Nakamura A, Okumura J, Muramatsu T. Quantitative analysis of transcriptional activity of viral and hybrid promoters in bovine preimplantation embryos. Molecular Reproduction and Development, 49: 368-373. 1998.
13) Nakamura A, Okumura J, Muramatsu T. Quick detection of firefly luciferase gene expression in live developing bovine embryos by photoncounting. Asian-Australasian Journal of Animal Science, 11: 498-502. 1998.
14) Nolan GP, Fiering S, Nicolas JF, Herzenberg LA. Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-D-galactosidase activity after transduction of Escherichia coli lacZ. Proceedings of National Academy of Sciences USA, 85: 2603-2607. 1988.
15) Peters KG, Rao PS, Bell BS, Kindman LA. Green fluorescent fusion proteins: powerful tools for monitoring protein expression in live zebrafish embryos. Developmental Biology, 171: 252-257. 1995.
16) SAS Institute. SAS User's Guide: Statistics. Version 5 edition, SAS Institute, Cary, NC. 1995.
17) Schnieke AE, Kind AJ, Ritchie WA, Mycock K, Scott AR, Ritchie M, Wilmut I, Colman A, Campbell KHS. Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts. Science, 278: 2130-2133. 1997.
18) Thompson EM, Adenot P, Tsuji FI, Renard J-P. Real time imaging of transcriptional activity in live mouse preimplantation embryos using a secreted luciferase. Proceedings of National Academy of Sciences USA, 92: 1317-1321. 1995.
19) Thompson JF, Hayes LS, Lloyd DB. Modulation of firefly luciferase stability and impact on studies of gene regulation. Gene, 103: 171-177. 1991.
20) Vilotte JL, Soulier S, Mercier JC, Gaye P, HueDelahaie D, Furet JP. Complete nucleotide sequence of bovine alpha-lactalbumin gene: comparison with its rat counterpart. Biochimie, 69: 609-620. 1987.

訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2008/03/10 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : 1) Bowen RA, Reed ML, Schnieke A, Shidel GE Jr, Stacey A, Thomas WK, Kajikawa O. Transgenic cattle resulting from biopsied embryos: expression of c-ski in a transgenic calf. Biology of Reproduction, 50: 664-648. 1994.
2) Burdon TG, Wall RJ. Fate of microinjected genes in preimplantation mouse embryos. Molecular Reproduction and Development, 33: 436-442. 1992.
3) Cousens C, Carver AS, Wilmut I, Colman A, Garner I, O'Neill GT. Use of PCR based methods for selection of integrated transgenes in preimplantation embryos. Molecular Reproduction and Development, 39: 384-391. 1994.
4) Eystone J, Jones JM, First NL. Some factors affecting the efficacy of oviduct tissue-conditioned medium for the culture of early bovine embryos. Journal of Reproduction and Fertility, 92: 59-64. 1991.
5) Hamano H, Kuwayama M. Effect of supplementing a culture medium with calf serum on in vitro maturation and development of bovine oocytes after in vitrofertilization. Journal of Reproduction and Technolo-gy, 14: 6-16 1992. (in Japanese)
6) Ikawa M, Kominami K, Yoshimura Y, Tanaka K,Nishimune Y, Okabe M. A rapid and non-invasiveselection of transgenic embryos before implantationusing green fluorescent protein (GFP). FEBS Letter,35: 125-128. 1995.
7) Janne J, Hyttinen J-M, Peura T, Tolvanen M, Alhohen L, Halmekyto M. Transgenic animals as bioproducers of therapeutic proteins. Annual Medicine, 24: 273-280. 1992.
8) Lin S, Yang S, Hopkins N. LacZ expression in germline transgenic zebrafish can be detected in living embryos. Developmental Biology, 161: 77-83. 1994.
9) Matsumoto K, Anzai M, Nakagata N, Takahashi A, Takahashi Y, Miyata, K. Onset of paternal gene activation in early mouse embryos fertilized with transgenic mouse sperm. Molecular Reproduction and Development, 39: 136-140. 1994.
10) Muramatsu T, Nakamura A. Production of transgenic cattle as an animal bioreactor: current status and perspective. Trends in Comparative Biochemistry and Physiology, 3: 9-21. 1997.
11) Nakamura A, Okumura J, Muramatsu T. Early screening of gene expression of SV40 driven lacZ introduced into bovine embryos. Asian-Australasian Journal of Animal Science, 8: 449-454. 1995.
12) Nakamura A, Okumura J, Muramatsu T. Quantitative analysis of transcriptional activity of viral and hybrid promoters in bovine preimplantation embryos. Molecular Reproduction and Development, 49: 368-373. 1998.
13) Nakamura A, Okumura J, Muramatsu T. Quick detection of firefly luciferase gene expression in live developing bovine embryos by photoncounting. Asian-Australasian Journal of Animal Science, 11: 498-502. 1998.
14) Nolan GP, Fiering S, Nicolas JF, Herzenberg LA. Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-D-galactosidase activity after transduction of Escherichia coli lacZ. Proceedings of National Academy of Sciences USA, 85: 2603-2607. 1988.
15) Peters KG, Rao PS, Bell BS, Kindman LA. Green fluorescent fusion proteins: powerful tools for monitoring protein expression in live zebrafish embryos. Developmental Biology, 171: 252-257. 1995.
16) SAS Institute. SAS User's Guide: Statistics. Version 5 edition, SAS Institute, Cary, NC. 1995.
17) Schnieke AE, Kind AJ, Ritchie WA, Mycock K, Scott AR, Ritchie M, Wilmut I, Colman A, Campbell KHS. Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts. Science, 278: 2130-2133. 1997.
18) Thompson EM, Adenot P, Tsuji FI, Renard J-P. Real time imaging of transcriptional activity in live mouse preimplantation embryos using a secreted luciferase. Proceedings of National Academy of Sciences USA, 92: 1317-1321. 1995.
19) Thompson JF, Hayes LS, Lloyd DB. Modulation of firefly luciferase stability and impact on studies of gene regulation. Gene, 103: 171-177. 1991.
20) Vilotte JL, Soulier S, Mercier JC, Gaye P, HueDelahaie D, Furet JP. Complete nucleotide sequence of bovine alpha-lactalbumin gene: comparison with its rat counterpart. Biochimie, 69: 609-620. 1987.

Right : 1) Bowen RA, Reed ML, Schnieke A, Shidel GE Jr, Stacey A, Thomas WK, Kajikawa O. Transgenic cattle resulting from biopsied embryos: expression of c-ski in a transgenic calf. Biology of Reproduction, 50: 664-648. 1994.
2) Burdon TG, Wall RJ. Fate of microinjected genes in preimplantation mouse embryos. Molecular Reproduction and Development, 33: 436-442. 1992.
3) Cousens C, Carver AS, Wilmut I, Colman A, Garner I, O'Neill GT. Use of PCR based methods for selection of integrated transgenes in preimplantation embryos. Molecular Reproduction and Development, 39: 384-391. 1994.
4) Eystone J, Jones JM, First NL. Some factors affecting the efficacy of oviduct tissue-conditioned medium for the culture of early bovine embryos. Journal of Reproduction and Fertility, 92: 59-64. 1991.
5) Hamano H, Kuwayama M. Effect of supplementing a culture medium with calf serum on in vitro maturation and development of bovine oocytes after in vitrofertilization. Journal of Reproduction and Technolo-gy, 14: 6-16 1992. (in Japanese)
6) Ikawa M, Kominami K, Yoshimura Y, Tanaka K,Nishimune Y, Okabe M. A rapid and non-invasiveselection of transgenic embryos before implantationusing green fluorescent protein (GFP). FEBS Letter,35: 125-128. 1995.
7) Janne J, Hyttinen J-M, Peura T, Tolvanen M, Alhohen L, Halmekyto M. Transgenic animals as bioproducers of therapeutic proteins. Annual Medicine, 24: 273-280. 1992.
8) Lin S, Yang S, Hopkins N. LacZ expression in germline transgenic zebrafish can be detected in living embryos. Developmental Biology, 161: 77-83. 1994.
9) Matsumoto K, Anzai M, Nakagata N, Takahashi A, Takahashi Y, Miyata, K. Onset of paternal gene activation in early mouse embryos fertilized with transgenic mouse sperm. Molecular Reproduction and Development, 39: 136-140. 1994.
10) Muramatsu T, Nakamura A. Production of transgenic cattle as an animal bioreactor: current status and perspective. Trends in Comparative Biochemistry and Physiology, 3: 9-21. 1997.
11) Nakamura A, Okumura J, Muramatsu T. Early screening of gene expression of SV40 driven lacZ introduced into bovine embryos. Asian-Australasian Journal of Animal Science, 8: 449-454. 1995.
12) Nakamura A, Okumura J, Muramatsu T. Quantitative analysis of transcriptional activity of viral and hybrid promoters in bovine preimplantation embryos. Molecular Reproduction and Development, 49: 368-373. 1998.
13) Nakamura A, Okumura J, Muramatsu T. Quick detection of firefly luciferase gene expression in live developing bovine embryos by photoncounting. Asian-Australasian Journal of Animal Science, 11: 498-502. 1998.
14) Nolan GP, Fiering S, Nicolas JF, Herzenberg LA. Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-D-galactosidase activity after transduction of Escherichia coli lacZ. Proceedings of National Academy of Sciences USA, 85: 2603-2607. 1988.
15) Peters KG, Rao PS, Bell BS, Kindman LA. Green fluorescent fusion proteins: powerful tools for monitoring protein expression in live zebrafish embryos. Developmental Biology, 171: 252-257. 1995.
16) SAS Institute. SAS User's Guide: Statistics. Version 5 edition, SAS Institute, Cary, NC. 1995.
17) Schnieke AE, Kind AJ, Ritchie WA, Mycock K, Scott AR, Ritchie M, Wilmut I, Colman A, Campbell KHS. Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts. Science, 278: 2130-2133. 1997.
18) Thompson EM, Adenot P, Tsuji FI, Renard J-P. Real time imaging of transcriptional activity in live mouse preimplantation embryos using a secreted luciferase. Proceedings of National Academy of Sciences USA, 92: 1317-1321. 1995.
19) Thompson JF, Hayes LS, Lloyd DB. Modulation of firefly luciferase stability and impact on studies of gene regulation. Gene, 103: 171-177. 1991.
20) Vilotte JL, Soulier S, Mercier JC, Gaye P, HueDelahaie D, Furet JP. Complete nucleotide sequence of bovine alpha-lactalbumin gene: comparison with its rat counterpart. Biochimie, 69: 609-620. 1987.

訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

Early screening of preimplantation bovine embryos was attempted by detecting firefly luciferase gene expression as bioluminescence. No detrimental effect was observed in a 3hr-exposure of 2-cell embryos to 500μM luciferin-containing medium on the subsequent embryonic development to the blastocyst stage, whereas a 6-hr exposure significantly reduced the percentageof the embryos that successfully developed to blastocysts (P<0.05). When the firefly luciferase gene driven by the SV 40 early enhancer and promoter was microinjected in the pronucleus of one-cell stage embryos, clear and strong bioluminescence signals were observed in embryos of four-to 16-cell stages within 10min. The presence of the DNA sequences encoding bovine α-lactalbumin secretion signal significantly enhanced the bioluminescence compared with the basic luciferase gene without the signal sequences (P<0.01). These results suggest that the secretion segnal sequences of bovine α-lactalbumin gene may improve the usefulness of the firefly luciferase reporter gene as a selection means of preimplantation embryos carrying transgenes.

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