Correlation Between Reactive Oxygen Species Scavenging Ability by ESR Spin Trap Method and Brightness of Miso

Abstract

In this study, we evaluated the scavenging abilities of three reactive oxygen species (hydroxyl radical (・OH), superoxide radical (O₂^-), and singlet oxygen (¹O₂)) using the ESR spin trap method for 15 types of miso with different lightness. The positive values of the scavenging abilities of all the reactive oxygen species (・OH, O₂^-, and ¹O₂) examined indicated that they exhibited good scavenging abilities, among which the scavenging ability of ・OH (4001 to 9074 mmol mannitol eq./g) was higher. The scavenging abilities of ・OH (R=-0.81) and O₂^- (R=-0.91) were negatively correlated with the brightness of miso, whereas the scavenging ability of ¹O₂ (R=0.81) was positively correlated with the same parameter. Miso samples with the lower brightness exhibited high ・OH and O₂^- scavenging abilities, and those with the higher brightness showed low ¹O₂ scavenging ability.