The purpose of this study was to investigate the effects of heat treatment on the properties of wheat starch. We heated wheat starch samples with five different emulsifier solutions: three different varieties of monoacylglyceroles (glycerin monostearate (MS), glycerin monooleate (MO), and glycerin monolinolate (ML)), and two varieties of monoacyldiglyceroles (DS and DO). The samples were heated at 150 °C for 0.5 h, 200 °C for 0.5 h, or 200 °C for 1.0 h, respectively. The heated starch samples in emulsifier solutions were thoroughly washed with chloroform and completely dried. Little change in the shapes of starch granules in all the samples were observed by scanning electron microscopy (SEM) images. X-ray diffraction (XRD) patterns of all starch samples, except for the sample heated in DS, displayed A-type patterns. However, there was disappearance of crystalline pattern in the starch samples heated in DS solution. The starches heated in an emulsifier showed less enthalpy change (endothermic peak 1) than the raw wheat starches, by differential scanning calorimetry (DSC). Starch samples heated in MS and DO showed more enthalpy changes (endothermic peak 2) than the raw wheat starch samples as measured by DSC. The samples heated in emulsifiers showed lower λmax values and lower blue value than the raw wheat starch samples by iodine color reaction. Moreover, the samples heated in emulsifier solutions showed smaller high molecular peak in size exclusion chromatography (SEC) profiles than the raw wheat starch. However, the extents of changes in peak intensities widely varied among different emulsifier species. These results suggested that amylopectin present in starch samples was decomposed during the heat treatment, and the degree of the decomposition differed based on the emulsifier species used. The susceptibility of starch samples to glucoamylases was higher among emulsifier-heated samples than raw wheat starch samples. However, the extent of decomposition of starch samples heated in monoacylglycerols is not similar to that of the starch samples heated in monoacyldiglycerols. These results suggest that the decomposition of starch samples heated in emulsifiers was not only affected by the heat treatment condition, but that other factors, such as the emulsifier hydrophilic-lipophilic balance (HLB), and the degree of unsaturation of the constituent fatty acids, also play a key role.
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