Determination of Some Nucleotides in Fish Muscle by Micellar Electrokinetic Capillary Chromatography

Abstract

The determination of nucleotides in foodstuffs has been usually undertaken by the absorption spectrochemical analysis in an ultraviolet region after the separation of these compounds by ion exchange chromatography. In this study, micellar electrokinetic capillary chromatography (MECC)was applied for this determination.
MECC was used to separate some nucleotides (adenosine-5'-triphosphate, adenosine-5'-diphosphate, adenosine-5'-mono-phosphate, inosine-5'-monophosphate, inosine and hypoxanthine)contained in the muscle of fish, and it became clear that this method was useful not only for the separation of these compounds but also for rapid determination (in less than 45 min). Nucleotides in fish muscle were extracted with 10% perchloric acid.
In conclusion, MECC is a rapid and efficient technique for resolution of nucleotides from fish muscle. This measurement can be used to determine the nucleotides of fish.