Abstract
The chromatographic behavior of manno-oligosaccharides derived from Saccharomyces cerevisiae mannan on two kinds of HPLC columns, an aminopropyl-silica column or a graphitized carbon column (GCC), was investigated. The order of elution of manno-oligosaccharides on both columns with acetonitrile–water was almost the same, that is, the retention increased with increasing molecular size. However, the GCC made it possible to isolate completely two isomers of mannotrioses (M3-1 and M3-2) with different linkage positions. We reinvestigated the structures of mannobiose (M2), M3s, and mannotetraose (M4) that were completely isolated by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and NMR spectroscopy.
