Structural Elucidation of Novel Phosphocholine-Containing Glycosylinositol-Phosphoceramides in Filamentus Fungi: (2). Spectral Analysis of the Sugar-Inositol Portion by 2D-NMR

Junko Yamada-Hada; Noriyasu Hada; Kazuhiro Aoki; Kenji Yamamoto; Tadahiro Takeda

doi:10.1248/cpb.52.473

Notes

Structural Elucidation of Novel Phosphocholine-Containing Glycosylinositol-Phosphoceramides in Filamentus Fungi: (2). Spectral Analysis of the Sugar-Inositol Portion by 2D-NMR

Junko Yamada-Hada, Noriyasu Hada, Kazuhiro Aoki, Kenji Yamamoto, Tadahiro Takeda

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Keywords: Acremonium sp., glycosylinositol-phosphoceramide, ¹H–³¹P HMQC, spectral analysis

JOURNAL FREE ACCESS

2004 Volume 52 Issue 4 Pages 473-476

DOI https://doi.org/10.1248/cpb.52.473

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Abstract

The sugar-inositol portion of the novel glycosylinositol-phosphoceramides, ZGL1 and ZGL2, from the filamentus fungi, Acremonium sp., were elucidated by a combination of NMR techniques including ¹H–¹H (COSY and HOHAHA) and ¹H–¹³C (HMQC and HMBC) spectroscopy. Further, examination of the ¹H–³¹P HMQC spectrum showed connectivity of inositol and ceramide through phosphate.

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