Purification and Characterization of New Special Ginsenosidase Hydrolyzing Multi-Glycisides of Protopanaxadiol Ginsenosides, Ginsenosidase Type I

Abstract

In this paper, the new type ginsenosidase which hydrolyzing multi-glycosides of ginsenoside, named ginsenoside type I from Aspergillus sp.g48p strain was isolated, characterized and generally described. The enzyme molecular weight was about 80 kDa. Ginsenosidase type I can hydrolyze different glycoside of protopanaxadiol type ginsenosides (PPD); i.e., can hydrolyze the 3(carbon)-O-β-glucoside of Rb₁, Rb₂, Rb₃, Rc, Rd; can hydrolyze 20(carbon)-O-β-glucoside of Rb₁, 20-O-β-xyloside of Rb₃, 20-O-α-arabinoside(p) of Rb₂ and 20-O-α-arabinoside(f) of Rc to produce mainly F₂, compound-K (C-K) and small Rh₂, but can not hydrolyze the glycosides of protopanaxatriol type ginsenoside (PPT) such as Re, Rf, Rg₁. So, when the ginsenosidase type I hydrolyzed ginsenosides, the enzyme selected ginsenoside-aglycone type, can hydrolyze different glycosides of PPD type ginsenoside; however no selected glycoside type, can hydrolyze multi-glycosides of PPD type ginsenosides. These properties were novel properties, and differentiated with the other previously described glycosidases.