Peroxidase-Like Catalytic Activity of Water-Insoluble Complex Linked Fe(III)-Thiacalix[4]arenetetrasulfonate with Tetrakis(1-methylpyridinium-4-yl)porphine via Ionic Interaction

Abstract

A new water-insoluble Fe³⁺-TCAS[4]/TMPyP complex linked tetraanionic Fe(III)-thiacalix[4]arenetetrasulfonate (Fe³⁺-TCAS[4]) with tetracationic tetrakis(1-methylpyridinium-4-yl)porphine (TMPyP) via ionic interaction was prepared. The peroxidase-like catalytic activity of the Fe³⁺-TCAS[4]/TMPyP complex was investigated based on the dye formation reaction by oxidation of 4-aminoantipyrine and phenol with H₂O₂ catalyzed by peroxidase. This Fe³⁺-TCAS[4]/TMPyP complex showed the highest activity in pH 5.5 acetate buffer solutions, and it was applied to the photometric determination of trace amounts of H₂O₂. The calibration curve was linear over the range from 1.0 to 35 μg of H₂O₂ in a 1.0 ml sample solution. Moreover, the method using glucoseoxidase and the Fe³⁺-TCAS[4]/TMPyP complex was applied to the determination of glucose, and the results were satisfactory even in control sera. The Fe³⁺-TCAS[4]/TMPyP complex can be applied to a practical sample, such as blood or urine, as an analytical reagent for the photometric determination of H₂O₂ in place of peroxidase.