Synthesis of ¹³C-Lidocaine as a Probe of Breath Test for the Evaluation of Cytochrome P450 Activity

Abstract

¹³C-Labeled lidocaine, 2-di[1-¹³C]ethylamino-N-(2,6-dimethylphenyl)acetamide (1), was synthesized from [1-¹³C]acetic acid in six steps, as a probe for a breath test to evaluate in vivo cytochrome P450 activity. The measurement of ¹³CO₂ in breath was successfully performed following oral administration of ¹³C-lidocaine 1 to mice.