Synthetic models related to methoxalen and menthofuran -- CYP2A6 interactions. Benzofuran and coumarin derivatives as potent and selective inhibitors of CYP2A6

Abstract

Human microsomal CYP2A6 contributes extensively to nicotine detoxication but also activates tobacco-specific procarcinogens to mutagenic products. We prepared a series of benzofuran and coumarin derivatives that have inhibitory effects on the activity of human cytochrome P450 (CYP) 2A6. The reported compounds methoxalen and menthofuran had potent inhibitory effects on the activity of CYP2A6 with IC₅₀ values of 0.47 μ M and 1.27 μ M, respectively. Synthetic benzofuran (4-methoxybenzofuran: IC₅₀=2.20 μ M) and coumarin (5-methoxycoumarin: IC₅₀=0.13 μ M and 6-methoxycoumarin: IC₅₀=0.64 μ M) derivatives, which have more selective effects than those of methoxalen and menthofuran, exhibited comparable activities against CYP2A6. These compounds can be used as a lead compounds in the design of CYP2A6 inhibitor drugs to reduce smoking and tobacco-related cancers.