Triterpenoid saponins of Pulsatilla koreana root have inhibition effects of TNF-α secretion in LPS-induced RAW 264.7 cells

Abstract

In the present study, a new oleanane-type triterpenoid saponin, pulsatilloside F (1), along with 21 known compounds (2–22), were isolated from the root of Pulsatilla koreana. Their chemical structures were elucidated by mass, ¹H, ¹³C NMR, COSY, HMQC and HMBC spectroscopy. Anti-inflammatory effects of the compounds were evaluated in terms of inhibitory of tumor necrosis factor α (TNF-α) secretion in the lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophage cell line. Compounds 19 and 20 exhibited particularly inhibitory effects with respective IC₅₀ values of 0.32 and 0.65 μM. Compounds 1–4, 7 and 10–13 exhibited inhibitory effects with inhibition rates up to 41.55-73.76% at a concentration of 5 μM, respectively.