Triterpenoid Saponins of Pulsatilla koreana Root Have Inhibition Effects of Tumor Necrosis Factor-α Secretion in Lipopolysaccharide-Induced RAW264.7 Cells

Abstract

In the present study, a new oleanane-type triterpenoid saponin, pulsatilloside F (1), along with 21 known compounds (2–22), were isolated from the root of Pulsatilla koreana. Their chemical structures were elucidated by mass, ¹H-, ¹³C-NMR, correlation spectroscopy (COSY), heteronuclear multiple quantum coherence (HMQC) and heteronuclear multiple bond connectivity (HMBC) spectroscopy. Anti-inflammatory effects of the compounds were evaluated in terms of inhibitory of tumor necrosis factor α (TNF-α) secretion in the lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophage cell line. Compounds 19 and 20 exhibited particularly inhibitory effects with respective IC₅₀ values of 0.32 and 0.65 µm. Compounds 1–4, 7 and 10–13 exhibited inhibitory effects with inhibition rates up to 41.55–73.76% at a concentration of 5 µm, respectively.