Author's Organization：Institute of Pharmaceutical Sciences, Faculty of Medicine, Kyushu University Institute of Pharmaceutical Sciences, Faculty of Medicine, Kyushu University Institute of Pharmaceutical Sciences, Faculty of Medicine, Kyushu University
A sensitive spot test for active methylene compounds was established by adding sodium dihydrogen phosphate in the alkaline reaction mixture of either trinitrobenzene or picric acid. A blank color faded or diminished in this method, and the developed coloration increased wtih a few exceptions. The color and a limit of detection of many active methylene compounds were tabulated.
