Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
Metabolism of Drugs. XLIII. Comparison of Glucuronyl Transfer Activity between β-Glucuronidase and Uridine Diphosphate Transglucuronylase.
Hisao TsukamotoKeitaro KatoKazuo YoshidaKiyoshi Tatsumi
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1964 Volume 12 Issue 6 Pages 734-737

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Abstract
1. UDP-transglucuronylase catalyzed the transfer of the glucuronyl group from UDP glucuronic acid to phenolic compounds, such as 2-naphthol, p-cresol, p-nitrophenol, m-aminophenol, and p-aminosalicylic acid, but the glucuronyl transfer by β-glucuronidase from phenolphthalein β-D-glucuronide or p-nitrophenyl β-D-glucuronide to these phenolic compounds was not observed. 2. The transfer product by β-glucuronidase from p-nitrophenyl β-D-glucuronide to methanol was identified by paper chromatography, and percentage of glucuronyl transfer activity was also measured. This glucuronide transferred 24.2 per cent of the liberated glucuronic group to methanol.
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© The Pharmaceutical Society of Japan
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