Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
Estimation of Creatinine in Urine with 3, 5-Dinitrobenzenesulfonate
Toshiro MurataShoko EtoKiyomi YamatsuKuniko Sugiura
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1964 Volume 12 Issue 7 Pages 770-773

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Abstract
For the estimation of urinary creatinine, DNSK-reagent was used and the estimation was satisfactorily carried out under the conditions as follows ; 1) Sample urine is prepared by diluting with water to contain 10∼80γ per ml. of creatinine. 2) To 4 ml. of sample urine, each 0.5 ml. of 2% DNSK-reagent and 5% KOH solution is added, and after 25 minutes the color developed is estimated at 530 mμ.
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© The Pharmaceutical Society of Japan
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