Detection of Morphine in Urine. II. An Improved Method by Thin-Layer Chromatography Utilizing Potassium Platinum Iodide as the Reagent for Both Coloration and Fluorescence

Abstract

A simple and reliable identification method of morphine was developed by successive performance of color and fluorescent reactions with potassium platinum iodide reagent utilizing the same thin-layer chromatogram; after separation by thin-layer chromatography morphine could be identified as a violet-colored spot with above reagent and then reconfirmed as a blue fluorescent spot under ultraviolet light after the chromatogram was exposed to ammonia vapor. The fluorometric identification is based on the essentially same principal to that of Kupferberg, et al.^{1, 2)}, however the present method has certain advatages to identify morphine, especially excreted in urine, in respect of simplicity and reliability.