抄録
Biogenesis of oospolactone (I) and oosponol (II) from a fungus, Oospora astringenes were studied by using glucose-[1-14C], glucose-[6-14C], malonate-[2-14C], formate-[14C], and uniformly labelled glucose-[14C]. Degradation methods of the two metabolites were established and the progress of cultivation was also studied. The isotope experiments suggest that the skeletons of the two metabolites are synthesized from five C2-units according to the ''acetate-malonate condensation'' and one C1-unit. C1unit is incorporated as methyl-C (carbon 11) in I, on the other hand, as =CH- (carbon 9) which participates to form the lactone ring in II. It is interesting that these two isocoumarins from the same culture have the different origin of lactone ring.
