Fluorometric Determination of Pyridoxal-5-phosphate and Pyridoxal in Biological Materials. I. Procedure and Application.
1966 年 14 巻 5 号 p. 482-487
詳細
1966 年 14 巻 5 号 p. 482-487
A fluorometric method of determination of pyridoxal-5-phosphate (PALP) and pyridoxal (PAL) in blood or organ has been developed. This method is based on Bonavita reaction of KCN with either PALP or PAL, and found to be convenient and has remarkable sensitivity for PAL as compared with the enzymatic methods. The method is also applicable to animal experiment, though it is somewhat inferior to the enzymatic methods in the sensitivity for PALP.
