Fluorometric Determination of Pyridoxal-5-phosphate and Pyridoxal in Biological Materials. II. Specificity of the Method in Pyridoxal-5-phosphate Determination.
1966 年 14 巻 5 号 p. 488-491
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1966 年 14 巻 5 号 p. 488-491
The specificity of the previously reported fluorometric method of determination of PALP in biological materials was investigated. The fluorescence excitation and emission spectra, the change in fluorescence intensity with pH, the electrophoresis, the paper chromatography, and the hydrolysis with acid phosphatase were examined with the PALP fraction obtained from mouse liver. The results showed that the method had high specificity for the determination of PALP in liver.
