Abstract
Biliary metabolites of o-aminoazotoluene (OAT), i. e. 4-amino-3, 2'-dimethylazobenzene, were investigated in rat and identified as N-glucuronide of OAT, N-glucuronide of OAT-derivative whose 2'-methyl group was oxidized hydroxymethyl, conjugates of 4'-hydroxylated OAT with sulfuric acid or glucuronic acid, similar conjugates of 4'-hydroxylated and N-acetylated OAT, and a double conjugate of 4'-hydroxylated OAT with N-glucuronic acid and O-sulfuric acid. The method for determination of OAT-NG, a main metabolite, in which an internal standard substance was adopted to thin-layer chromatography, was examined and gave good results. Similar methods were also examined for determination of other minor metabolites.
