Studies on the Metabolic N-Demethylation. V. Effect of Phenobarbital on the Oxidative Demethylation in Vitro in the Liver of Adrenalectomized Rats

Abstract

The activities of demethylating enzymes were decreased by adrenalectomy, but were stimulated by the administration of phenobarbital or cortisone in the liver of adrenalectomized rats. Incorporation of ¹⁴C-labeled amino acid into liver microsomal proteins of adrenalectomized rats was apparently increased by the administration of phenobarbital. The magnitude of phenobarbital-effect was decreased by pretreatment with puromycin and 8-azaguanine in intact animals.