Thiamine Derivatives of Disulfide Type. I. Formation of Thiamine from Thiamine Propyl Disulfide in Rat Intestine in Vitro

Abstract

The conversion of thiamine from thiamine propyl disulfide was studied in vitro using rat intestine set at a modified Wiseman and Smyth's apparatus. The following conclusions were drawn from the experimental results obtained. 1) It was found that thiamine propyl disulfide was converted to free thiamine in mucosal solution and that propyl disulfide appeared in intestinal tissues during the conversion. 2) The formation of thiamine in mucosal and serosal solutions followed the first order kinetics apparently and were same magnitude. 3) The conversion may be considered as the coupled reactions between chemical and enzymic systems and the participation of glutathione reductase to this conversion was expected.