Abstract
In order to obtain a large amount of fusarenon-X, a cytotoxic mycotoxin of Fusarium nivale Fn 2B, environmental factors influencing the production of the toxin were investigated with the following results. 1) With the stationary culture of F. nivale on Czapek medium, the supplemented peptone or yeast extract increased the lethal toxicity of culture filtrate to mice. 2) Peptone-supplemented Czapek medium (PSC medium) provided all necessary ingredients for the high level production of fusarenon-X. 3) With PSC medium, the maximal yield of fusarenon-X was observed 6-8 days of cultivation at 27°, and the yield decreased at lower temperature. 4) Rice, barley, wheast and corn supported the production of the toxin, and the highest toxicity was observed with rice grains.
