Abstract
Alkaline proteinases, named alkinonase A and AF, are isolated from the cultured broth of Streptomyces violaceorectus. Both alkinonases show remarkably strong antiinflammatory activity for the carrageenin-induced edema. Alkinonase A and AF are belonging to a new type of alkaline proteinases of microbial origin. The effect of inhibitors on the proteolytic activity and the substrate specifities of alkinonases are resembled to that of the neutral proteinases of microbial origin and different from that of the alkaline proteinases of microbial origin. Production, isolation, purification, effect of pH on the proteolytic activity, pH stability, effect of temperature on the proteolytic activity, effect of inhibitors on the proteolytic activity, substrate specifities and anti-inflammatory activity for the carrageenin induced edema of alkinonases are described in this paper.
